. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. the Molecular Devices SPECTRAmax® 190 and 250. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range. Opaque-walled 96- or 384-well tissue culture plates are available as white or black with solid bottoms and white or black with clear bottoms. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 0200-6262. Important things to consider when purchasing a multimode microplate reader. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Features. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. It features fully-automated kinetic reads of the 96-well plate via the WinKQCL TM Software. President of Molecular Devices. It has a 340-750nm Spectral Range. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. minutes and begin reading microplates with seven mouse clicks. Please refer to the Obsolete Microplate Reader Models for more information. 00. This study investigated the efficacy of fermented seaweed (Eucheuma cottonii) on the remediation of fluoride-contaminated soil. $3,450. Instrument settings can. The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. 96- and 384-well. This robust system is capable of reading 96 wells, each with its own sample beam and reference detector. Specifications subject to change without notice. 00 . Glass microfiber: Use for cell harvesting and binding assays, liquid scintillation counting or high loading, DNA binding and purification; Melt blown polypropylene: Use for pre-filtration; Cellulose acetate: Use for low protein binding,. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Recertification service. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. The StakMax stacker also allows users to feed plates into the loading area manually. 5 pM Luminescence performance Wavelength range 300–850 nm Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. % transmittance of plates used in a plate reader. These microplates have a corner notch for use in standard plate readers. Reader Documentation Version No. 96- and 384-well. x. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Turn the instrument around so that the back of the instrument is. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Please click on the links below to view the Microplate Reader Comparison Tables. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. Get Quote . 384-WELL AND 96-WELL 10MM-HEIGHT STERILE MICROPLATES. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Categories: Plate Readers. Order Now. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. x. 96-well plates - Filter plates with 0. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. Supersedes 1. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Next-generation luminescence plate reader. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. Top or bottom detection is available with a simple click of a button. High-throughput, automated dynamic light scattering. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. For the 96-well plate, this equates to 1 fg per well to 1 ng per well; for the 384-well plate it is 0. CO 2 & O 2 control. com. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 1 x plates; Instrument dimensions. The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. x. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits. Microplate washers are designed for effortless use with 96- or 384-well plates in routine ELISA applications. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. This is equivalent to having 511 built-in filters,The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. 96- and 384-well microplates. Deep well blocks available in 24, 96 and 384-well formats An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Description Bottom Volume Qty/Pk Qty/Cs 3635 96 Well UV-Plate Flat 370 µL 2550per well of this semi-solid mixture was pipetted into 6-well plates. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Capabilities. Uncontrolled Copy for Training and Reference Purposes Only . The CellTiter-Glo assay was used to measure ATP. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The luminescence was measured in a SpectraMax. µl) was added to each well and incubated at 37°C for 1 h. 9000-0756. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. SoftMax Pro GxP Software version 5. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Absorbance cuvette port. It also integrates with the. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. Multimodal plate reader: Yes: 25 mm: Yes—Max. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Versa Max. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. These models can no longer be repaired. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. Plate Background OD, which was used for subsequent tests, was measured as follows. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Microplate Readers include the M3, M4, M5, and M5e models. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. No. Unparalleled performance on a personalized platform. Growth medium was then replaced with fatty acid uptake solution. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Suggested plates are black walled. one 96-well sample plate can be controlled from 15–40°C,. The procedures in this protocol support the 96-well reaction plate. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. M5. Infinite possibilities. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. The system can perform readings. . FAST AND ACCURATE METERTECH Microplate Reader $3,650. 000 to 2. 5 µL of each sample was pipetted into a 96-well plate for PCR. Create custom fluorescence assays without having to purchase expensive filter sets. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible. Plus 384. Figure 1. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. #ABS PLUS) Methods. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). Bay Gray powder coated metal back plate, column, and base. Fluorescence Microplate Reader Comparison Chart. 25, 3. Optional 16-way manifold. 2% in. 000 – 4. The variations include adjustable. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 5 second read per plate. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. Compact designs make these instruments. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume differences that may. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. 1 fmol luciferase per well. Sample by eye within . Compare this item. x. Optimized for fluorescence microplate readers. Or scan up to 384 wells at any time to determine the best assay conditions. x. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. This can increase throughput and efficiency, thus saving time and space in the lab. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Features. Part Number. Align the two slots on the bottle holder with the two pegs on the rail. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hourMicroplate Reader, For Lab. Supersedes 1. Accurate, efficient nucleic acid quantitation is critical for molecular biology labs that work with precious, rare, or difficult-to-process samples and applications requiring precise measurement. Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. SpectraDrop High-Throughput Screening (HTS) Kit. x. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. Page 1 of 8 . D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. The entire Bulk Kit is sufficient for 100 plates (96- and 384-well), or 70 plates (1536-well). Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. adjust the reader. • Photometric range: 0. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. 2. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. Shaking: Linear, orbital, and double orbital. #E010) Pyroplate 96-well microplate (ACC cat. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 5% at 1. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. 1 OD at 230 nm. 96-, 384-, and 1536-well microplates. Related Products: 96 Well Plate Reader. 0 . Industry standard, 96-well plates. The soil was amended with either 1. Return to top. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Erik Koppes, Richard Haas, Jerry Vockley, Lina Ghaloul-Gonzalez approximately 5,000 cells were seeded in 4 replicates in 96-well plates. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. It offers extremely low absorbance background, excellent. The rate of molecular motion of Protein G slows down when it is bound to antibodies, resulting in an increase of the FP value (Figure 2). x. 0 . Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. 384-WELL SBS STERILE MICROPLATE. $3,207. Through absorbance readings from both the ultraviolet and visible spectrum range, it can determine the concentration of samples, conduct positive or negative detection of antibodies and. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Enter the name of the plate type i. 0% ± 0. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. Instrument settings can beIntegrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. Access this by going to. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). These robust readers have been placed in labs from. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Turn on Plate Reader and wait for it to complete its start-up routine. Your applications, your modes, your choice. Microplate optimization and read height optimization were performed for best results. 25% and 0. Exposure of ApNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε = 13,500 at 382 nm). Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. Four wash/rinse bottles are included and configurable for both 96- and 384. B. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 0. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). Endpoint, kinetic, spectrum. x. 0025 OD from 0. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. the use of a reader calibrated with primary NIST and NMI standards. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. 00. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Polypropylene deep well plates, round bottom. Microplate Readers. 130009WMicroplate Readers. Plate Shaking. Automated live cell imaging – cell counting and confluence. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. Click Add to create the plate. Top and bottom reads are. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. Dark blue indicates well will be read. Microplate Reader. 1c shows a cover designed to perfuse 8 wells in series on a standard 96-well microplate, as well as a cover for the entire standard 96-well microplate. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Bioz Stars score: 86/100, based on 40 PubMed citations. QuickSync. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. as well as all other Molecular Devices' microplate readers. Running both standard spectrophotometer and microplate. 0200-6263. and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. * Note: Programming the washer with a final aspirate height of 4. From the dropdown Menu, select Wellplates. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. 7°C and the bottom to 36. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . Temperature control: 4°C above ambient to 45°C. The same volume of genotyping mixture (2x KASP Master mix plus KASP Assay mix) was added to the each well. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. A. No accessories are needed and SoftMax® Pro Software. 001 ABS (displayed), 0. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Published Dec 16,. Related products. C. Molecular Spectroscopy Consumables & Accessories Thermal Analysis. In SoftMax Pro, Dual Read mode was selected. SpectraMax ID5 Plate Reader (Molecular Devices). It also integrates with the powerful SoftMax Pro Software for. 3. FlexIII. This approach enables uniform light dose application to 9 wells (a 3-by-3 matrix in a 96-well plate) simultaneously, which can be leveraged to shorten the duration of PDT experiments (Fig. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 2 pM 384–well: 3 pM 96–well: 2 pM 384–well: 4 pM 1 0200-7012POS Glow for 96-, 384-, and 1536-well plates Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. Learn how you can utilize microplate readers to drive landmark discoveries. Contact our support team with your questions regarding Molecular Devices products and applications. 1. 000 to 2. explore more. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. 96-, 384-, and 1536-well microplates. Cross-talk <0. )Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. VIS absorbance detection. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. For biochemical assays, the washer removes unbound material and unreacted reagents. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. Anticancer drug assay was also conducted using conventional 96-well plates as controls. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. x. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Materials. Absorbance equation. 90349). 4 (Molecular Devices cat. Washer maintenance kit: 2 X T 2. Integrated stacker option. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. Source plate capacity; 1 x plates. No accessories are needed and SoftMax® Pro Software. Service Contracts, Preventative Maintenance and. An additional reference channel for optimizing the light intensity; Auto switch-off of the light source when not in use to extend lifetime; An automated self-check of major components at instrument start-upThe EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Absorbance was then determined at 450 nm by SpectraMax Paradigm microplate reader (Molecular. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis. M5. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Published Dec 16, 2020 | Updated Dec 06, 2021. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Uncontrolled Copy for Training and Reference Purposes Only . A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. 2. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. When samples are added to the wells, the Protein G molecules are resuspended and binding occurs. x. for molecular biology. FlexIII. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Refurbished. 340 PC384. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. What are the dimensions of the plate? Code. Fluorescence Microplate Reader Comparison Chart. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Microplate format. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. Microplate Readers include the M3, M4, M5, and M5e models. (one set per assay) to clear 96-well microplates. These robust readers have been placed in labs from Antarctica to. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 190. Two high-energy xenon flash lamps: Reading capabilities. Molecular Devices: FlexStation 3: Multimodal: Limited - monochronometer based: N/A:the iD5 reader’s filter system. . Select models. Two different agonists, acetylcholine and carbachol, were tested.